■Before using this software, activate the Java™ and Java Script function of the web browser.

This software is specifically for designing the primer sets for Loop-mediated Isothermal Amplification (LAMP) method.

This software generates the primer sets based on the target sequence information, which meets the primer designing requirements. One primer set contains 4 primers, FIP (Forward Inner Primer), F3, BIP (Backward Inner Primer) and B3. FIP (BIP) consists of the sequence of the F1c (B1c) and F2 (B2) regions. F1, F2, F3 are about 20bp long sequences selected from the target gene, B1, B2, B3 are about 20bp long sequences selected from the complementary strand. F1c and F1, B1 and B1c are complementary regions. The position of primers on the target gene is as follows:

This software can also design the loop primer, which is located between F1c and F2c, or B1c and B2c.